Centro de Documentação da PJ
Monografia
 27116 | (Biologia) |  |
DNA typing protocols : molecular biology and forensic analysis / Bruce Budowle.. [et al.].- Natick, MA : Eaton Publishing, cop. 2000.- vii, 304 p. : il. ; 26 cm. - (BioForensic Sciences Series)
ISBN 1-881299-23-6
CIÊNCIA FORENSE, GENÉTICA, MARCADOR GENÉTICO, ADN, ANÁLISE LABORATORIAL
Chapter 1 - Introduction. Chapter 2 - Sample collection and storage. Chapter 3 - Extraction of DNA. Chapter 4 - Determination of the quantity of DNA. Chapter 5 - Restriction fragment-length polymorphism typing of variable number tandem repeat loci. Chapter 6 - PCR-based analyses: allele-specific oligonucleotide assays. Chapter 7 - Electrophoretic separation and allele detection by silver staining for the Loci DS80 and amelogenin. Chapter 8 - Short tandem repeat loci typing using polyacrylamide gel electrophoresis and decoupled fluorescent scanner detection. Chapter 9 - Capillary electrophoresis for short tandem repeat loci typing and real time fluorescent detection. Chapter 10 - PCR-based analysis: mithocondrial DNA sequencing. Appendix 1 - Quality assurance standards. Appendix 2 - Allele frequency data. Appendix 3 - Reagents and supplies.