Centro de Documentação da PJ
Analítico de Periódico
CD 327
Sensitive method for the confident identification of genetically variant reptides in human hair keratin [Recurso eletrónico] / Zheng Zhang M.D. ..[et al.]
Journal of Forensic Sciences, Vol. 65, n. 2 (March 2020), 24 p.
Ficheiro de 928 KB, em formato PDF.
ADN, ANÁLISE DE CABELO, ANÁLISE LABORATORIAL
Materials and methods. Human hair sample preparation. Direct extration method. Modified NaOH - based SDS Repeated extraction method. Hair reptide mass spectral library construction including Published GVPs. Spectrum library searching. Sequence database searching. Proteomic methods. Results and discussion. Identification of Hair Proteome including cuticular keratins by direct extraction methods. Overall gel identification. Major and minor gel band identification. GVP panel analyses in all ten tractions and among three methods. Comparison between the direct method and modified NaOH + SDS method. Sensitivity. Reproducibility. Examination of artifacts among tree methods. Identification of hair proteome and cuticular keratins from as little as 1-cm-long human hair shaft by direct extraction method. Examination of the direct method in another donor. Summary and conclusions.
