Centro de Documentação da PJ
Monografia
 CD258 |
Development and validation of standard operating procedures for measuring microbial populations for estimating a postmortem interval [Documento electrónico] / Jeffrey K. Tomberlin ..[et al.].- [Rockville, MD] : National Criminal Justice Reference Service (NCJRS), 2014.- 1 CD-ROM ; 12 cm
Research report submitted to the U.S. Department of Justice. NIJ Award Number: 2010-DN-BX-K243. Ficheiro de 13,4 MB em formato PDF (82 p.).
IDENTIFICAÇÃO BIOLÓGICA, ANÁLISE LABORATORIAL, ANÁLISE DE VESTÍGIOS, IDENTIFICAÇÃO DE CADÁVER
Predicting the postmortem interval of a decedent is a major task of law enforcement. Most
methods implemented by death investigators rely on qualitative information (i.e., rigor mortis,
livor mortis). Microbes represent 99% of somatic cells in and on a human body. No data are
available on the use of these organisms to predict the time since death of a decedent, though it is
known that certain chemicals, many of which are likely a result of microbial communities, are
released by decomposing remains in a reliable pattern. Moreover, the effects of microbes on
insect colonization of remains, sometimes the best predictor of a postmortem interval, are not
understood. Because of a lack of understanding of microbial succession on decomposing human
remains, no standard operating procedures (SOP) for sampling and using this information have
been developed and validated. We developed a SOP for sampling and pyrosequencing bacteria
communities on human remains to predict the actual time since death. We conducted a series of
laboratory and field studies to achieve this end. The field studies will implement the SOP for
sampling bacteria on decomposing human remains at the Forensic Anthropology Research
Facility at Texas State University.