Centro de Documentação da PJ
Analítico de Periódico
CD 341
Phenibut screening and quantification with liquid chromatography–tandem mass spectrometry and its application to driving cases [Recurso eletrónico] / Marek Dziadosz ..[et al.]
Journal of Forensic Sciences, Vol. 69, n. 2 (March 2024), p. 725-729
Ficheiro de 410 KB em formato PDF.
ANÁLISE DE SANGUE, ANÁLISE DE DROGA, ESTUPEFACIENTE, SUBSTÂNCIA PSICOTRÓPICA
An analytical strategy for identification by an LC–MS/MS multitarget screening method and a suitable LC–MS/MS based quantification were developed for the psychotropic drug phenibut. The samples analyzed were collected during traffic control and were associated with driving under the influence of drugs. A positive sample for phenibut was identified in a single case of driving under the influence. The quantification revealed a drug concentration of 1.9 ìg/mL. An interaction with blood alcohol (BAC = 0.10%) was discussed as the explanation of the way of driving and deficit manifestations observed (swaying, nystagmus, quivering of the eyelid, and reddened eyes). According to the available information, the quantified phenibut concentration could be explained by an intake of four tablets (self-reported) during the day containing 250 mg of the drug. Chromatography was performed with a Luna 5 ìm C18 (2) 100 A, 150 mm × 2 mm analytical column, and a buffer system consisted of 10 mM ammonium acetate and 0.1% acetic acid (v/v) included in mobile phases marked as A (H2O/methanol = 95/5, v/v) and B (H2O/methanol = 3/97, v/v). An effective limit of detection (LOD = 0.002 ìg/mL) could be achieved for the multitarget screening method. The quantification of phenibut was performed on a second LC–MS/MS system with LOD/LOQ values of 0.22/0.40 ìg/mL. Since phenibut quantification data are rare, the presented information can be used with caution for evaluation of positive cases in the future.
